Hiyoshi Corporation Provides CALUX®Assay
  Mechanism of CALUX Cell   Accuracy   Analysis Process
  HOMEMethodAccuracy
 
 
 
 
Determinate the relative dioxin concentration of unknown samples by comparison of their ability to induce luciferase activity to that of luciferase activity induced by a series of TCDD standards.    
   
 
 
 
 
   
【3.5g soil, ash and other solid samples】1.0pgTEQ/g
【3.5L water and other liquid samples】1.0pgTEQ/L
【3.5m3N exhaust gas and other gas samples】 1.0pgTEQ/m3N
 
 
PRECISION TEST
  Quality and accuracy of the assay is determined by multiple replicate analysis of standard solutions.
Standard Solution Conc. Standard Solution Conc. (Repeat 6 Times)
(pg/ml)
125.00
62.50
31.25
15.63
7.81
3.91
1.95
0.98
0.49
Average±S.D.(pg/ml) C.V.(%)
53.07 ± 26.51
39.21 ± 12.53
23.76 ± 4.48
13.27 ± 2.32
7.44 ± 0.29
4.11 ± 0.24
2.17 ± 0.24
0.99 ± 0.17
±
49.96
31.96
18.88
17.47
3.90
5.93
10.90
16.89
 
Cross Reactivity
One reason CALUX has a high correlation with chemicals analysis is because the relative potency values (REPs) of the dioxin-like HAHs in the CALUX Assay are close to the WHO-TEF values.
Compounds
Reactivity
CALUX-TEF(REP50%) WHO-1988 TEF WHO-2006 TEF
P
C
D
D
s
2,3,7,8-TCDD
1,2,3,7,8-PeCDD
1,2,3,4,7,8-HxCDD
1,2,3,6,7,8-HxCDD
1,2,3,7,8,9-HxCDD
1,2,3,4,6,7,8-HpCDD
OCDD
1 1 1
0.73 1 1
0.075 0.1 0.1
0.098 0.1 0.1
0.061 0.1 0.1
0.031 0.01 0.01
0.00034 0.0001 0.0003
P
C
D
F
s
2,3,7,8-TCDF
1,2,3,7,8-PeCDF
2,3,4,7,8-PeCDF
1,2,3,4,7,8-HcCDF
1,2,3,6,7,8-HxCDF
1,2,3,7,8,9-HxCDF
2,3,4,6,7,8-HxCDF
1,2,3,4,6,7,8-HpCDF
1,2,3,4,7,8,9-HxCDF
OCDF
0.067 0.1 0.1
0.14 0.05 0.03
0.58 0.5 0.3
0.13 0.1 0.1
0.14 0.1 0.1
0.11 0.1 0.1
0.31 0.1 0.1
0.024 0.01 0.01
0.044 0.01 0.01
0.0016 0.0001 0.0003
D
L

P
C
B
s
3,3',4,4'=TCB(#77)
3,4,4',5-TCB(#81)
3,3',4,4',5-PeCB(#126)
3,3',4,4',5,5'-HxCB(#169)
2,3,3',4,4'-PeCD(#105)
2,3,4,4',5-PeCD(#114)
2,3',4,4',5-PeCD(#118)
2',3,4,4',5-PeCD(#123)
2,3,3',4,4',5-HxCB(#156)
2,3,3',4,4',5'-HxCB(#157)
2,3,4,4',5,5'-HxCB(#167)
2,3,3',4,4',5,5'-HpCB(#189)
0.0014 0.0001 0.0001
0.0045 0.0001 0.0003
0.038 0.1 0.1
0.0011 0.01 0.03
0.0000010 0.0001 0.0003
0.00014 0.0005 0.0003
0.0000010 0.0001 0.0003
0.00000030 0.0001 0.0003
0.00014 0.0005 0.0003
0.0000030 0.0005 0.0003
0.00000030 0.00001 0.0003
0.00000020 0.0001 0.0003
 

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